Improved Method for Determining Soybean Phospholipid Composition by Two-dimensional TLC-phosphorus Assay
نویسندگان
چکیده
منابع مشابه
Improved semiautomated method for assay of phosphorus in serum phospholipids.
A dependable automated method for the analysis of phospholipids would be highly desirable, because manual proceduresfortheir assay are long and tedious. One such procedure has been proposed by Whitley and Album foruse with an AutoAnalyzer (Technicon Instruments Corp., Tarrytown, N.Y. 10591). The chemical basis of this system is sound, and has been des#{243}ribed in detail (1). However, the meth...
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Add 0.5 ml of serum dropwise with constant shaking to a mixture of 4 ml of absolute alcohol and 2 ml of diethyl ether in a 10-ml volumetric flask. Carefully heat the mixture in a hot water bath until it begins to boil, cool, and dilute to 10 ml with absolute alcohol. Mix and filter. Evaporate 4 ml of the filtrate (equivalent to 0.2 ml of serum), 2 ml at a time, in a 15 X 2.5 cm test tube marked...
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ژورنال
عنوان ژورنال: Journal of Japan Oil Chemists' Society
سال: 1992
ISSN: 1884-2003
DOI: 10.5650/jos1956.41.1029